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hypoxia chamber  (Baker Company)


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    Structured Review

    Baker Company hypoxia chamber
    Hypoxia Chamber, supplied by Baker Company, used in various techniques. Bioz Stars score: 97/100, based on 1701 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hypoxia+chamber/InvivO2/pm42190812-37-24-26
    Average 97 stars, based on 1701 article reviews
    hypoxia chamber - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    other:

    Article Title: Spatial blueprint of colorectal cancer identifies Hypoxic CAF program that orchestrates Go-or-Grow decisions and predicts outcomes.
    Article Snippet: The tumor microenvironment (TME) critically influences colorectal cancer (CRC) progression, but its spatial organization and functional impact remain incompletely understood.. We integrated single-cell transcriptomics (412,208 cells) with spatial transcriptomics (76,627 spots from 38 sections of 32 patients) to deconstruct the CRC microenvironment, employing computational approaches for niche identification, SCENIC network inference, and cell–cell communication analysis.. Key findings were validated in primary CAF cultures, Seahorse metabolic analysis, and the TCGA cohort.

    Article Title: Chiral Gd-DOTA as a Versatile Platform for Hepatobiliary and Tumor Targeting MRI Contrast Agents.
    Article Snippet: The leakage of gadolinium ions (Gd3+) from commercial Gd3+-based contrast agents (GBCAs) in patients is currently the major safety concern in clinical magnetic resonance imaging (MRI) scans, and the lack of task-specific GBCAs limits its usage in the early detection of disease and imaging of specific biological regions.. Herein, ultrastable GBCAs were constructed via decorating chiral Gd-DOTA with a phenylic analogue to one of the pendent arms, and the stability constant was determined as high as 27.08, accompanied by negligible decomplexation in 1 M of HCl over 2 years.. A hepatic-specific chiral Gd-DOTA was screened out as a potential alternative to commercial Gd-EOB-DTPA, while combination with functional molecules favored chiral Gd-DOTA as tumor targeting probes.

    Article Title: The N6-methyladenosine demethylase ALKBH5 regulates the hypoxic HBV transcriptome
    Article Snippet: Hypoxic treatment of cells was carried out in a hypoxic incubator (New Brunswick Galaxy 48R, Eppendorf) or a hypoxia chamber (InvivO 2 , Baker-Ruskinn Technologies) at 5% CO 2 and 1% oxygen.

    Article Title: Targeting C2 reduces ischemia-reperfusion injury-induced complement activation in preclinical human models
    Article Snippet: Hypoxia was induced by culturing the cells in a hypoxia chamber (Ruskinn INVIVO2 500, Baker-Ruskinn) at 0.1% O 2 and 5% CO 2 at 37 °C for 24h (primary ECs) or 48h (HUVEC).

    Article Title: Mitochondrial Double-Stranded RNA Triggers Ferroptosis via PKR–ISR Signaling in Renal Ischemia–Reperfusion Injury
    Article Snippet: To simulate IR injury, HK-2 cells were subjected to H/R by incubating for 6 hours in a hypoxic environment (1% O 2 , 5% CO 2 , and 94% N 2 ) using serum- and glucose-free media, which had been saturated overnight prior to media exchange in a hypoxia chamber (InvivO 2 , Baker Co.), followed by reoxygenation by replacing with standard media and returning cells to a normoxic incubator for the designated recovery time before harvesting.

    Article Title: P53 – a new player in the metabolic adaptation of colorectal carcinoma cells under hypoxia
    Article Snippet: Hypoxic conditions were achieved using a hypoxia chamber (InvivO 2 400, Baker Ruskin).

    Irradiation:

    Article Title: Autophagy is the main driver of radioresistance of HNSCC cells in mild hypoxia
    Article Snippet: .. Unless where specifically mentioned, hypoxia refers to 1% oxygen for 16 h prior to irradiation and which was performed using the INVIVO 2 400 hypoxia chamber (Baker Ruskinn, Bridgend, UK). .. For siRNA knockdowns, cells were cultured in 35 mm dishes for 24 h until 30%–50% confluent, and then incubated with 2.5 μL of Lipofectamine RNAiMAX transfection reagent (Life Technologies, Paisley, UK), in the presence of 40 nM of either HIF‐1α, HIF‐1β and Beclin 1 siRNA, or 4 nM of either BNIP3 and BNIP3L siRNA for 48 h. The following siRNA sequences were used: Qiagen all‐stars negative control siRNA (Qiagen, Southampton, UK) as the non‐targeting control (NTC); ON‐TARGET PLUS siRNA pools against HIF‐1α and HIF‐1β from Horizon Discovery (Cambridge, UK), BECLIN 1 siRNA (5′‐CAGUUUGGCACAAUCAAUA‐3′) from Eurogentec (Seraing, Belgium).

    Incubation:

    Article Title: HIF-1α Drives Distinct Aspects of Hypoxia-Induced Glucose Metabolism in Intestinal Epithelial Cells.
    Article Snippet: For non-tracer studies, Caco-2 intestinal epithelial cells (ATCC®, HTB-37TM) were 99 cultured in DMEM containing 4.5 g/L glucose, 2 mM L-glutamine, 10% heat-inactivated 100 FBS, 1% MEM non-essential amino acids, and 1% penicillin–streptomycin (100 U/mL) 101 and maintained in a humidified atmosphere of ambient air, supplemented with 5% CO2 102 and maintained at 37 °C. .. For hypoxic exposures, cells were incubated in pre-103 equilibrated tracer/control media at 1% O2, 5% CO2 and maintained at 37°C in a 104 humidified hypoxia chamber (Ruskinn, InvivO2 500). ..



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    ( A ) Oxygen levels (PO 2 (mmHg)) in cell culture media under control conditions and following exposure to hypoxia for 24 hr in <t>the</t> <t>C-chamber</t> <t>hypoxia</t> <t>sub-chamber</t> (Biospherix; unpaired t -test, p<0.0001); ( B ) Hierarchical clustering, using Ward’s criterion, of the first three principal components of the normalized gene expression profiles of all 17,777 genes passing quality control shows clear separation of 12 and 24 hr hypoxia-exposed samples versus control and from reoxygenated samples. Bar charts: mean ± s.e.m.
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    BioSpherix normobaric hypoxia chambers
    ( A ) Oxygen levels (PO 2 (mmHg)) in cell culture media under control conditions and following exposure to hypoxia for 24 hr in <t>the</t> <t>C-chamber</t> <t>hypoxia</t> <t>sub-chamber</t> (Biospherix; unpaired t -test, p<0.0001); ( B ) Hierarchical clustering, using Ward’s criterion, of the first three principal components of the normalized gene expression profiles of all 17,777 genes passing quality control shows clear separation of 12 and 24 hr hypoxia-exposed samples versus control and from reoxygenated samples. Bar charts: mean ± s.e.m.
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    Image Search Results


    ( A ) Oxygen levels (PO 2 (mmHg)) in cell culture media under control conditions and following exposure to hypoxia for 24 hr in the C-chamber hypoxia sub-chamber (Biospherix; unpaired t -test, p<0.0001); ( B ) Hierarchical clustering, using Ward’s criterion, of the first three principal components of the normalized gene expression profiles of all 17,777 genes passing quality control shows clear separation of 12 and 24 hr hypoxia-exposed samples versus control and from reoxygenated samples. Bar charts: mean ± s.e.m.

    Journal: eLife

    Article Title: Adrenomedullin restores the human cortical interneurons migration defects induced by hypoxia

    doi: 10.7554/eLife.108134

    Figure Lengend Snippet: ( A ) Oxygen levels (PO 2 (mmHg)) in cell culture media under control conditions and following exposure to hypoxia for 24 hr in the C-chamber hypoxia sub-chamber (Biospherix; unpaired t -test, p<0.0001); ( B ) Hierarchical clustering, using Ward’s criterion, of the first three principal components of the normalized gene expression profiles of all 17,777 genes passing quality control shows clear separation of 12 and 24 hr hypoxia-exposed samples versus control and from reoxygenated samples. Bar charts: mean ± s.e.m.

    Article Snippet: For these experiments, we induced hypoxia using the C-chamber hypoxia sub-chamber (Biospherix).

    Techniques: Cell Culture, Control, Gene Expression